AVS2015 Session SM-ThP: Surface Modification of Materials by Plasmas for Medical Purposes Poster Session
Thursday, October 22, 2015 6:00 PM in Hall 3
SM-ThP-1 Replacing Self-Assembled Monolayers by Functional Plasma Polymers in Fabrication of Immunosensors
Lenka Zajickova, Anton Manakhov, Ekaterina Makhneva, David Kovar (Masaryk University, Czech Republic); Gleb Dorozinsky, Olena Shynkarenko, Gennadii Beketov (Lashkaryov Institute of Semiconductor Physics, Ukraine); Petr Skladal (Masaryk University, Czech Republic)
Plasma polymerization is an efficient way to the modifications of various surfaces by desired functional groups. It finds potential applications in tissue engineering or fabrication of biosensors. This work reports on the plasma polymerization of two functional coatings that were used as a replacement of self-assembled monolayers (SAMs) in the fabrication of immunosensors. Amine-based coatings were deposited from cyclopropylamine mixed with argon in low pressure capacitively coupled discharge whereas carboxyl-based coatings were prepared by co-polymerization of maleic anhydride and acetylene in atmospheric pressure dielectric barrier discharge. The coatings were prepared on gold surfaces of either quartz crystal microbalance or surface plasmon resonance sensors. Their performance was tested on a model system, monoclonal antibody (Ab AL-01) against human serum albumin (HSA) interacting with the corresponding HSA antigen. The immobilization of antibodies and inactivation of free non-reacted groups was realized in buffers with various pH. A crucial point was the stability of the prepared plasma polymers in liquids that was studied by time-varying sensor response and also by physical characterization methods (X-ray photoelectron spectroscopy, infrared spectroscopy, atomic force microscopy, ellipsometry) in the dry state. The performance of the immunosensors with the plasma polymer layers were compared to the sensors prepared using the standard procedure using SAM.
SM-ThP-2 Microstructure and Biocompatibility Evaluation of Zirconia Coatings Grown by Plasma Electrolytic Oxidation Technique
Bih-Show Lou (Chang Gung University,Taiwan, Taiwan, Republic of China); Shao-Fu Lu (National Taipei University of Technology, Taiwan, Taiwan, Republic of China); Jyh-Wei Lee (Ming Chi University of Technology, Taiwan, Taiwan, Republic of China); Yung-Chin Yang (National Taipei University of Technology, Taiwan, Taiwan, Republic of China)
The plasma electrolytic oxidation (PEO) technique has been widely studied and applied in industries due to its ability to create functional oxide layers on light metals and Zr alloys. In this work, the zirconi a coatings were grown on pure Zr metals by PEO treatment. Different duty cycle values were applied in K3PO4 aqueous solution containing various concentrations of KOH to fabricate zirconia coatings. The detailed microstructure of zirconia coating was studied through the scanning electron microscope and transmission electron microscope. The PEO treated zirconia coating consisted of a thin continuous barrier layer and a thick porous outermost layer, which consisted of mainly monoclinic and minor tetragonal ZrO2 phases. The corrosion resistances of zirconia films in Hank’s solution were evaluated. The biocompatibility of zirconia coatings were further examined using 3T3 and MG63 cells. Effects of duty cycles and KOH concentrations on the microstructures and biocompatibilities of zirconia coatings were discussed in this study.