AVS2009 Session BM-ThP: BioMEMS Poster Session

Thursday, November 12, 2009 6:00 PM in Hall 3

Thursday Evening

Time Period ThP Sessions | Topic BM Sessions | Time Periods | Topics | AVS2009 Schedule

BM-ThP-2 Boundary Slip and Nanobubble Study in Micro/Nanofluidics with Atomic Force Microscope
Yuliang Wang, Bharat Bhushan (The Ohio State University)

The boundary condition at the liquid-solid interface in micro/nano scale is an important issue in micro/nanofluidics systems. Recent studies have shown that the fluid velocity near solid surfaces is not equal to the velocity of the solid surface on hydrophobic surfaces, which is called boundary slip. The degree of boundary slip is evaluated by a slip length. Theoretical and experimental studies suggest that at the solid-liquid interface, the presence of nanobubbles is responsible for the breakdown of the no-slip condition. Nanobubbles are long lasting on hydrophobic surfaces, and movement and coalescence of nanobubbles are observed with higher scan loads during imaging with tapping mode AFM.

The slip length can be measured with both contact atomic force microscopy (AFM) and dynamic AFM methods. In the contact AFM method, the slip length is obtained by fitting the measured hydrodynamic force applied to a sphere as a function of separation distance between the sphere and solid surfaces when the sphere approaches the surfaces. In the dynamic AFM method, the amplitude and phase shift data of an oscillating sphere are recorded during approach to sample surfaces at low velocities. These data are then used to get the hydrodynamic damping coefficient to obtain the slip length. Until now, slip length has generally been studied on hydrophobic surfaces with AFM. The boundary slip properties of superhydrophobic surfaces are seldom studied. The impact of surface roughness on the obtained slip lengths also needs to be eliminated for superhydrophobic surfaces. Moreover, because the sphere should disturb the nanobubble during approach to sample surfaces in both the contact and dynamic AFM method, a new technique is needed to evaluate boundary slip. Regarding nanobubbles, the current studies mainly focus on their physical properties. The interaction between nanobubbles and the surfaces supporting them is seldom studied. More importantly, the relationship between nanobubble immobility and surface properties should be studied.

In this study, both contact and dynamic AFM methods have been applied to study the boundary slip on hydrophilic, hydrophobic, and superhydrophobic surfaces. A new AFM based technique is proposed to study boundary slip. Nanobubble movement and coalescence, as well as tip-bubble interaction, are studied in detail. The physical interaction between nanobubbles and the surfaces supporting them is investigated. Moreover, the relationship between nanobubble immobility and surface properties of hydrophobic surface is revealed.

BM-ThP-4 Chemical and Morphological Properties of Amino-Silane Coated Surfaces for DNA Purification
Lorenza Marocchi, Lorenzo Lunelli, Laura Pasquardini, Cristina Potrich, LiaEmanuela Vanzetti (FBK-CMM, Italy); Graziano Guella (University of Trento, Italy); Cecilia Pederzolli (FBK-CMM, Italy); Mariano Anderle (Provincia Autonoma di Trento, Italy)

DNA purification and PCR amplification are a requirement for most genetic analysis. Combining these processes in a single micro device minimizes sample loss and contamination problems as well and reduces time and costs of analysis. Different strategies are available to perform DNA extraction on a chip. Here we exploited amino-coated silicon and pyrex surfaces as a tool for specific binding of DNA through the electrostatic interaction between amino groups and nucleic acids. Amino groups have been introduced on the surfaces via silanization carried out in wet condition [1] using three silanes carrying a different number of amino groups and different alkoxy groups ( (3-Aminopropyl)triethoxysilane (APTES), (3-Aminopropyl)trimethoxysilane (APTMS) and (3-[2-(2-Aminoethylamino)ethylamino]propyl-trimethoxysilane (AEEA) ). The influence of different silanization conditions on surface properties, such as homogeneity and thickness of the silane layer, was also studied by changing solvents, concentration of silane solution and reaction temperature. The kinetic of hydrolysis of the alkoxy groups followed by oligomerization of aminosilanes was characterized by NMR measurements. Amino-coated surfaces were characterized by AFM, XPS and absorption spectroscopy to define their chemical and morphological properties. Multi-amino silane were found less prone to form uniform and tiny layers than mono-amino silanes, resulting less suitable for successive PCR amplification.

Finally, we analyzed the ability of treated surfaces to selectively adsorb/desorb genomic DNA with the aim to purify DNA from unwanted cellular components. Preliminary results suggest this strategy as very promising, permitting to obtain a considerable yield of purified DNA in short time.

[1] Fiorilli, S.; Rivolo, P.; Descrovi, E.; Ricciardi, C.; Pasquardini, L.; Lunelli, L.; Vanzetti, L.; Pederzolli, C.; Onida, B. & Garrone, E. (2008), Journal of Colloid and Interface Science 321, 235-241.
Time Period ThP Sessions | Topic BM Sessions | Time Periods | Topics | AVS2009 Schedule