ICMCTF2016 Session D2-1: Surface Coatings, Micro/Nano Texturing, Nanotubes, Drug Delivery, Biodegradable Implants

Monday, April 25, 2016 10:00 AM in Room Sunrise

Monday Morning

Time Period MoM Sessions | Abstract Timeline | Topic D Sessions | Time Periods | Topics | ICMCTF2016 Schedule

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10:00 AM D2-1-1 Investigation of In-Vitro Properties of NiTi After MAO Coatings
Suleyman Sukuroglu (Gumushane University, Turkey); Yasar Totik (Ataturk University, Turkey); EbruEmine Sukuroglu (Gumushane University, Turkey); Ersin Arslan, Ihsan Efeoglu (Ataturk University, Turkey)

This study was focused on the coating of TiO2 layer by using the method of Micro Ark Oxidation with the aim of the improvement of surface and mechanical properties of Shaped-memory and bio material NiTi alloy. In vitro ability was investigated by soaking the coated NiTi samples in simulated body fluid (SBF) at temperature 37 °C for various time periods. After soaking, Ni+2 release was applied to the media where coated and uncoated shape-memory NiTi samples are available, and its control was made by means of ICP-MS device. In-vitro cytotoxicty tests of coated and uncoated samples were made by means of XTT test. The results show that the bioactivity and bioadaptation properties on aggressive surfaces such as body fluid contacted are increased by the TiO2 coating. The results also show that Toxic and carcinogenic effects of Ni+2 release is significantly reduced by the TiO2 coating. Thus, it is observed that the properties that limit the use of NiTi alloys has been substantially fixed by MAO coating.

10:20 AM D2-1-2 Surface Modification of Biodegradable Electrospun Scaffolds using Plasma Discharge with Sputter Deposition of a Titanium Target
Danila Petlin (Griffith University, Australia); Sergei Tverdokhlebov, Evgeniy Bolbasov, Evgeniy Shesterikov, Nadezhda Danilenko (Tomsk Polytechnic University, Russian Federation); Yulia Khodyrevskaya, Larisa Antonova, Vera Matveeva, Elena Velikanova, Yulia Kudryavtseva (Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”, Russian Federation); Yuri Anissimov (Griffith University, Australia); Leonid Barbarash (Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”, Russian Federation)

A critical aspect in the development of materials for biomedical applications is the optimization of their surface properties to achieve an adequate cell response. In the present work, biocompatible scaffolds were prepared by electrospinning solutions of poly-L-lactide (PLLA) and poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV). The electrospun scaffolds were modified by plasma of magnetron discharge with titanium (Ti) target sputtering in argon atmosphere. The influence of the plasma treatment time on morphology, chemical composition, crystalline structure and mechanical properties of the scaffolds was investigated. Also, in vitro experiments on cultured human endothelial hybrid cell line EA-hy 926 were conducted in order to study the effect of plasma treatment over cell adhesion and viability. It was demonstrated that plasma treatment can enhance the biocompatibility of the fibrous scaffolds.

10:40 AM D2-1-3 Thin Films for Biomedical Applications: from Sensors to Electrodes
Joel Borges, Marco Rodrigues, Diogo Costa (Minho University, Portugal); Albano Cavaleiro (University of Coimbra, Portugal); Filipe Vaz (Minho University, Portugal)
Noble metal nanoparticles (NPs) such as gold or silver have been used for many centuries, providing different colours in the windows of the medieval cathedrals and in ancient Roman glasses. Nowadays, the interest in nanocomposite materials containing noble NPs embedded in TiO2, Al2O3, etc., is related with their potential use for a wide range of advanced technological applications, namely electrodes and sensors for a wide range of biomedical driven applications [1].

Most of these applications rely on the so-called Localized Surface Plasmon Resonance (LSPR) absorption, which is governed by the type of the noble metal nanoparticles, NPs, their distribution, size and shape and as well as of the dielectric characteristics of the host matrix [2]. Since changes in size, shape and distribution of the clusters are fundamental parameters for tailoring the LSPR effect, a set of films with a wide range of noble metal concentration was prepared and the optical responses analyzed in detail. The films were deposited by DC magnetron sputtering and in order to promote the clustering of the NPs the as-deposited samples were subjected to an in-air annealing protocol. Results show that the clustering of metallic NPs affects the optical response spectrum and, for certain volume fractions, it broadens when the polarizable NPs are non-randomly distributed in the matrix. This will be the starting point to study the application of such thin film system in a wide range of bio-sensing applications.

The microstructural changes (structure, grain size and surface morphology) of the films promoted by heat-treatment seems to rule the overall biological response, as it will be shown by the interaction of the thin films with well-known proteins such as Bovine Serum Albumin (BSA), as well as with microbial cells (C. albicans). In fact, preliminary results showed that the BSA adhesion is dependent on surface nanostructure morphology, which in turn depends on the annealing temperature that changed the roughness and wettability of the films. The thin films also induced a significant alteration of the microbial cell membrane integrity, and ultimately the cell viability, which in turn affected the adhesion on its surface.

11:20 AM D2-1-5 Comparison Study on the Mechanical Property and Biocompatibility of W-contained Ti-based and Zr-based Thin Film Metallic Glasses
Po-Chi Wang (National Taipei University of Technology, Taiwan, Republic of China); Jyh-Wei Lee (Ming Chi University of Technology, Taiwan, Republic of China); Yung-Chin Yang (National Taipei University of Technology, Taiwan, Republic of China); Bih-Show Lou (Chang Gung University, Taiwan, Republic of China)
Thin film metallic glasses (TFMGs) have been reported to exhibit unique properties different from conventional crystalline metallic films. They have high strength, large elastic limits, excellent corrosion and wear resistance due to their amorphous structure. Among several kinds of material systems of TFMG, the W-contained TFMGs have not yet to be explored extensively. In this work, quaternary Zr-based Zr-Ti-Si-W and Ti-based Ti-Zr-Si-W TFMGs were fabricated on Si wafer and 316L stainless steel disk substrates by a four targets co-sputtering system. The TiN coating was also fabricated as a reference. The amorphous phase of TFMG was determined by the X-ray diffractometer (XRD). The microstructures of thin films were examined by the field-emission scanning electron microscopy (FE-SEM). A nanoindenter was used to evaluate the hardness of TFMGs. The scratch test and HRC-DB adhesion tester were employed to evaluate the adhesion properties of coatings. The anti-corrosion properties of TFMGs and TiN were evaluated by the potentiodynamic polarization tests in 3.5 wt.% NaCl aqueous solution. The MG-63 cell line (human osteosarcoma) was used to investigate cell-material interaction and biocompatibility of these coatings. Comparison study on the microstructure, mechanical property, corrosion resistance and biocompatibility of W-contained Zr-based and Ti-based TFMGs were discussed in this work.
11:40 AM D2-1-6 Camphor DLC Films Deposition in Polyurethane Prevents Candida albicans Biofilm
Thaisa B. Santos, Everton Santos, Angela Vieira, Polyana Alves Radi, Sônia Khouri, Homero Maciel, Rodrigo Pessoa (Universidade do Vale do Paraiba - UNIVAP- Brazil); Lucia Vieira (Institute of Research and Development –IPD/ UNIVAP and Tecnological Institute of Aeronautics, ITA/LPP, Brazil)

Camphor was incorporated into diamond-like carbon (DLC) films to prevent growth of candida albicans yeasts on polyurethane substrates, which is a material commonly used for catheter manufacturing. The camphor:DLC film for this investigation was produced by plasma enhanced chemical vapor deposition (PECVD), using an apparatus based on the flash evaporation of organic liquid (hexane) containing diluted camphor. The film was deposited at low temperature less than 25 °C. We obtained very adherent camphor:DLC films that accompanied the substrate flexibility without delamination. The camphor:DLC films were characterized by Raman spectroscopy, scanning electron microscopy, and atomic force microscopy. Biofilms of candida yeasts were cultivated in vitro and where developed on polyurethane, with and without camphor:DLC, to verify fungicide properties of DLC. In addition, colony-forming units (CFU /mL) were used to measure the number of microorganisms on the surface of the sample. The camphor:DLC films prevented growth of candida biofilms better than bare polyurethane. This resulted in 99.0% and 91.0% protection for camphor:DLC and DLC, respectively, when compared to polyurethane, which was totally contaminated. These results open the doors to studies of DLC coatings with fungicide properties deposited in polymers such as polyurethane used in the production of catheters or other biomedical applications.

Time Period MoM Sessions | Abstract Timeline | Topic D Sessions | Time Periods | Topics | ICMCTF2016 Schedule